The plates were incubated at 37✬/5% CO2 for the appropriate time (30 or 48 hours). To untreated control wells, 50 µL of medium only was added. After allowing the cells to re-attach overnight at 37✬/5% CO2, 50 µL of a 2x concentrated serial titration of trastuzumab emtansine was added to the appropriate replicate wells (n = 6). Figure Legend: In two separate assay plates, 50 µL/well of HER2+ SKBR3 cells (10,000/well) were plated in medium with 2x concentrated CellTox™ Green dye.
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